Mouse Anti-Chicken IgY-BIOT

Cat. No.
8320-08  
Size
0.5 mg  
Price (USD)
$305.00 
Clone G-1
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-BIOT
Immunogen Affinity purified chicken Ig or isolated lymphocytes
Specificity Chicken/Turkey IgY
Alternalte Name(s) Chicken IgG, Turkey IgG
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested 6-9
FLISA – Quality tested
Flow Cytometry – Reported in literature 10-13
Immunohistochemistry-Frozen Sections – Reported in literature 2-4
Immunocytochemistry – Reported in literature 5
Immunoprecipitation – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796497
  • ELISA plate was coated with purified chicken IgY and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Chicken IgY-BIOT (SB Cat. No. 8320-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

ELISA plate was coated with purified chicken IgY and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Chicken IgY-BIOT (SB Cat. No. 8320-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
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References
1. Chen CH, Lehmeyer JE, Cooper MD. Evidence for an IgD homologue on chicken lymphocytes. J Immunol. 1982;129:2580-5. (Immunogen, IP)
2. Javed MA, Frasca S Jr, Rood D, Cecchini K, Gladd M, Geary SJ, et al. Correlates of immune protection in chickens vaccinated with Mycoplasma gallisepticum strain GT5 following challenge with pathogenic M. gallisepticum strain Rlow. Infect Immun. 2005;73:5410-9. (IHC-FS)
3. Hansell C, Zhu XW, Brooks H, Sheppard M, Withanage S, Maskell D, et al. Unique features and distribution of the chicken CD83+ cell. J Immunol. 2007;179:5117-25. (IHC-FS)
4. Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (IHC-FS)
5. Singh S, Briles WE, Lupiani B, Collisson EW. Avian influenza viral nucleocapsid and hemagglutinin proteins induce chicken CD8+ memory T lymphocytes. Virology. 2010;399:231-8. (ICC)
6. Bailey JS, Rolón A, Hofacre CL, Holt PS, Wilson JL, Cosby DE, et al. Intestinal humoral immune response and resistance to Salmonella challenge of progeny from breeders vaccinated with killed antigen. Intl J Poult Sci. 2007;6:417-23. (ELISA)
7. Fasina YO, Holt PS, Moran ET, Moore RW, Conner DE, McKee SR. Intestinal cytokine response of commercial source broiler chicks to Salmonella typhimurium infection. Poult Sci. 2008;87:1335-46. (ELISA)
8. Lardinois A, van den Berg T, Lambrecht B, Steensels M. A model for the transfer of passive immunity against Newcastle disease and avian influenza in specific pathogen free chickens. Avian Pathol. 2014;43:118-24. (ELISA)
9. Orr-Burks N, Gulley SL, Gallardo RA, Toro H, van Ginkel FW. Immunoglobulin A as an early humoral responder after mucosal avian coronavirus vaccination. Avian Dis. 2014;58:279-86. (ELISA)
10. Del Cacho E, Gallego M, López-Bernard F, Sánchez-Acedo C, Lillehoj HS. Isolation of chicken follicular dendritic cells. J Immunol Methods. 2008;334:59-69. (FC)
11. Del Cacho E, Gallego M, Lillehoj HS, López-Bernard F, Sánchez-Acedo C. Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses. Vet Immunol Immunopathol. 2009;129:66-75. (FC)
12. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)
13. Petkov DI, Linnemann EG, Kapczynski DR, Sellers HS. Identification and characterization of two distinct bursal B-cell subpopulations following infectious bursal disease virus infection of White Leghorn chickens. Avian Dis. 2009;53:347-55. (FC)