Mouse Anti-Human CD11b-APC

Cat. No.
9546-11  
Size
100 tests  
Price (USD)
$260.00 
Clone ICRF44
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-APC
Immunogen Rheumatoid synovial cells and human monocytes
Specificity Human/Porcine/Rhesus/Cynomolgus/Chimpanzee/Baboon CD11b
Alternalte Name(s) Mac-1α, Mo-1, CR3, integrin αM, C3biR, ITGAM, CR3A, p170
Description CD11b is the 165 kDa αM chain which combines with the CD18 β2 integrin subunit to form the Mac-1 heterodimer. It is expressed on activated lymphocytes, monocytes, granulocytes and a subset of NK cells. CD11b functions in cell-cell and cell-substrate interactions and is a ligand for C3bi, CD50 (ICAM-3), CD54 (ICAM-1) and CD102 (ICAM-2).
Format/Conjugate APC (Allophycocyanin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 7,9
Immunohistochemistry-Frozen Sections – Reported in literature 3
Immunocytochemistry – Reported in literature 4
Immunoprecipitation – Reported in literature 1
Western Blot – Reported in literature 2
Blocking – Reported in literature 5,6
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796905
  • Human peripheral blood granulocytes were stained with Mouse Anti-Human CD11b-APC (SB Cat. No. 9546-11).

Human peripheral blood granulocytes were stained with Mouse Anti-Human CD11b-APC (SB Cat. No. 9546-11).
 
Related Products (6)
References
1. Feng C, Zhang L, Almulki L, Faez S, Whitford M, Hafezi-Moghadam A, et al. Endogenous PMN sialidase activity exposes activation epitope on CD11b/CD18 which enhances its binding interaction with ICAM-1. J Leukoc Biol. 2011;90:313-21. (IP)
2. David A, Kacher Y, Specks U, Aviram I. Interaction of proteinase 3 with CD11b/CD18 (β2 integrin) on the cell membrane of human neutrophils. J Leukoc Biol. 2003;74:551-7. (WB)
3. Mayer WJ, Klaproth OK, Hengerer FH, Kook D, Dirisamer M, Priglinger S, et al. In vitro immunohistochemical and morphological observations of penetrating corneal incisions created by a femtosecond laser used for assisted intraocular lens surgery. J Cataract Refract Surg. 2014;40:632-8. (IHC-FS)
4. Friedenberger M, Bode M, Krusche A, Schubert W. Fluorescence detection of protein clusters in individual cells and tissue sections by using toponome imaging system: sample preparation and measuring procedures. Nat Protoc. 2007;2:2285-94. (ICC)
5. Malhotra V, Hogg N, Sim RB. Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3). Eur J Immunol. 1986;16:1117-23. (Block)
6. Nasr AB, Haithcoat J, Masterson JE, Gunn JS, Eaves-Pyles T, Klimpel GR. Critical role for serum opsonins and complement receptors CR3 (CD11b/CD18) and CR4 (CD11c/CD18) in phagocytosis of Francisella tularensis by human dendritic cells (DC): uptake of Francisella leads to activation of immature DC and intracellular survival of the bacteria. J Leukoc Biol. 2006;80:774-86. (Block)
7. Lester EA, Babensee JE. Proinflammatory phenotype of endothelial cells after coculture with biomaterial-treated blood cells. J Biomed Mater Res A. 2003;64:397-410. (FC)
8. Hérodin F, Thullier P, Garin M, Drouet M. Nonhuman primates are relevant models for research in hematology, immunology and virology. Eur Cytokine Netw. 2005;16:104-16. (Chimpanzee, Baboon, Cynomolgus, Rhesus Reactivity)
9. Singh S, Kloss FR, Brunauer R, Schimke M, Jamnig A, Greiderer-Kleinlercher B, et al. Mesenchymal stem cells show radioresistance in vivo. J Cell Mol Med. 2012;16:877-87. (FC, Porcine Reactivity)