Goat Anti-Human IgG, Mouse ads-UNLB

Cat. No.
2044-01  
Size
1.0 mg  
Price (USD)
$110.00 
Specificity Reacts with the heavy chain of human IgG
Source Pooled antisera from goats hyperimmunized with human IgG
Cross Adsorption Human IgM and IgA; mouse immunoglobulins and pooled sera; may react with IgG from other species
Purification Affinity chromatography on human IgG covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-UNLB
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1
Flow Cytometry
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795671
  • ELISA plate was coated with purified human IgG, IgM, and IgA, and mouse IgG, IgM, and IgA (mouse Ig). Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Mouse ads-UNLB (SB Cat. No. 2044-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).

ELISA plate was coated with purified human IgG, IgM, and IgA, and mouse IgG, IgM, and IgA (mouse Ig). Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Mouse ads-UNLB (SB Cat. No. 2044-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
 
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References
1. Caven TH, Sturgill JL, Conrad DH. BCR ligation antagonizes the IL-21 enhancement of anti-CD40/IL-4 plasma cell differentiation and IgE production found in low density human B cell cultures. Cell Immunol. 2007;247:49-58. (ELISA)