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  • Goat Anti-Human IgM, Mouse/Bovine/Horse SP ads-UNLB

Goat Anti-Human IgM, Mouse/Bovine/Horse SP ads-UNLB

Cat. No.:
2023-01
Purified Goat Anti-Human IgM antibody with minimal reactivity to mouse, bovine, and horse serum proteins for use in ELISA assays.
$111.00
Size Price (USD) Quantity
1.0 mg $111.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-UNLB
Specificity Reacts with the heavy chain of human IgM
Source Pooled antisera from goats hyperimmunized with human IgM
Cross Adsorption Human IgG and IgA; mouse, bovine, and horse serum proteins (SP); may react with IgM from other species
Purification Method Affinity chromatography on human IgM covalently linked to agarose
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1-4
FLISA

RRID Number AB_2795619
Gene ID 3507 (Human)
Gene ID Symbol IGHM (Human)
Gene ID Aliases AGM1; MU; VH
UniProt ID P01871 (Human)
UniProt Name IGHM_HUMAN (Human)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

References and Citations

  1. 1. Magnani DM, Rogers TF, Beutler N, Ricciardi MJ, Bailey VK, Gonzalez-Nieto L, et al. Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques. Sci Transl Med. 2017;9:eaan8184. (ELISA)
  2. 2. Isho B, Abe KT, Zuo M, Jamal AJ, Rathod B, Wang JH, et al. Persistence of serum and saliva antibody responses to SARS-CoV-2 spike antigens in COVID-19 patients. Sci Immunol. 2020;5:eabe5511. (ELISA)
  3. 3. Honjo K, Russell RM, Li R, Liu W, Stoltz R, Tabengwa EM, et al. with humoral immunodeficiency. Cell Rep Med. 2020;2:100164. (ELISA)
  4. 4. Zhang B, Huo J, Huang Y, Teo SY, Duan K, Li Y, et al. mRNA booster vaccination enhances antibody responses against SARS-CoV2 omicron variant in individuals primed with mRNA or inactivated virus vaccines. Vaccines (Basel). 2022;10:1057. (ELISA)
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  1. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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  2. Ramos cell lysates treated with HM71224 and stimulated with Goat F(ab')<sub>2</sub> Anti-Human IgM-UNLB (SB Cat. No. 2022-01) were resolved by electrophoresis, transferred to PVDF membrane, and probed with anti-phospho-PLCγ2Y1217 followed by an HRP conjugated secondary antibody and chemiluminescent detection.<br/>Image from Kim Y, Park KT, Jang SY, Lee KH, Byun J, Suh KH, et al. HM71224, a selective Bruton's tyrosine kinase inhibitor, attenuates the development of murine lupus. Arthritis Res Ther. 2017;19:211. Figure 1(a)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  4. Human peripheral blood lymphocytes were stained with Goat Anti-Human IgM-BIOT (SB Cat. 2020-01) and Mouse Anti-Human CD19-PE (SB Cat. No. 9340-09) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-FITC (SB Cat. No. 6300-02).
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  6. ELISA plate was coated with serially diluted Human IgM Lambda-UNLB (SB Cat. No. 0158L-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  7. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  8. ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgM-UNLB (SB Cat. No. 9020-01) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-HRP (SB Cat. No. 1070-05).
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  11. FLISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-PE (SB Cat. No. 6157-09).
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  12. Frozen newborn mouse cartilage section was stained with Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01) followed by Mouse Anti-Goat IgG Fc-AF555 (SB Cat. No. 6158-32) and DAPI.
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  13. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa-UNLB (SB Cat. No. 2060-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  15. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type VI Collagen-UNLB (SB Cat. No. 1360-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT (SB Cat. No. 6300-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
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  16. ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgM-UNLB (SB Cat. No. 9022-01) followed by Goat Anti-Mouse IgG<sub>3</sub>, Human ads-HRP (SB Cat. No. 1100-05).
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  17. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-UNLB (SB Cat. No. 2070-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  18. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  19. Human peripheral blood lymphocytes were stained with Goat Anti-Human IgD-UNLB (SB Cat. 2030-01) and Mouse Anti-Human CD19-PE (SB Cat. No. 9340-09) followed by Swine Anti-Goat IgG(H+L), Human/Rat/ Mouse SP ads-FITC (SB Cat. No. 6300-02).
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