Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT

Cat. No.
4050-08  
Size
1.0 mg  
Price (USD)
$80.00 
Specificity Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM
Source Pooled antisera from goats hyperimmunized with rabbit IgG
Cross Adsorption Mouse and human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on pooled rabbit IgG covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 2.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-3
FLISA
Flow Cytometry 4-7
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 8-10
Immunohistochemistry-Paraffin Sections 6,11-13
Immunohistochemistry-Whole Mount 14,15
Immunocytochemistry 16-19
Western Blot 20-28
Separation 29
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2732896
  • ELISA plate was coated with purified rabbit IgG and IgM, human IgG, IgM, and IgA, and Mouse IgG, IgM, and IgA. Immunoglobulins were detected with Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4050-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
  • Chicken sperm was stained with anti-CaMKIV followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4050-08), Streptavidin-CY2 (SB Cat. No. 7100-21), and DAPI.

    Image from Nguyen TM, Combarnous Y, Praud C, Duittoz A, Blesbois E. Ca2+/calmodulin-dependent protein kinase kinases (CaMKKs) effects on AMP-activated protein kinase (AMPK) regulation of chicken sperm functions. PLoS One. 2016;11(1):e0147559. Figure 4(f)
  • Paraffin embedded mouse lamina propria section post sensitization with shrimp tropomyosin was stained with anti-Stat3phosphoTyr705 followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4050-08), HRP conjugated avidin, DAB, and hematoxylin.

    Image from Fu L, Peng J, Zhao S, Zhang Y, Su X, Wang Y. Lactic acid bacteria-specific induction of CD4+Foxp3+T cells ameliorates shrimp tropomyosin-induced allergic response in mice via suppression of mTOR signaling. Sci Rep. 2017;7:1987. Figure S8

ELISA plate was coated with purified rabbit IgG and IgM, human IgG, IgM, and IgA, and Mouse IgG, IgM, and IgA. Immunoglobulins were detected with Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4050-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 

Chicken sperm was stained with anti-CaMKIV followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4050-08), Streptavidin-CY2 (SB Cat. No. 7100-21), and DAPI.

Image from Nguyen TM, Combarnous Y, Praud C, Duittoz A, Blesbois E. Ca2+/calmodulin-dependent protein kinase kinases (CaMKKs) effects on AMP-activated protein kinase (AMPK) regulation of chicken sperm functions. PLoS One. 2016;11(1):e0147559. Figure 4(f)
 

Paraffin embedded mouse lamina propria section post sensitization with shrimp tropomyosin was stained with anti-Stat3phosphoTyr705 followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4050-08), HRP conjugated avidin, DAB, and hematoxylin.

Image from Fu L, Peng J, Zhao S, Zhang Y, Su X, Wang Y. Lactic acid bacteria-specific induction of CD4+Foxp3+T cells ameliorates shrimp tropomyosin-induced allergic response in mice via suppression of mTOR signaling. Sci Rep. 2017;7:1987. Figure S8
 
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