Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-BIOT

Cat. No.
6440-08  
Size
1.0 mg  
Price (USD)
$95.00 
Specificity Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM
Source Pooled antisera from donkeys hyperimmunized with rabbit IgG
Cross Adsorption Mouse, rat, and human serum proteins (SP); may react with immunoglobulins from other species and the light chains of other rabbit immunoglobulins
Purification Affinity chromatography on rabbit IgG covalently linked to agarose
Isotype Donkey IgG
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 2.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1
FLISA
Other referenced applications for relevant formats include -
Flow Cytometry 2,3
Immunohistochemistry-Paraffin Sections 4,5
Immunocytochemistry 6
Western Blot 7,8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796366
  • Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit IgG-UNLB isotype control (SB Cat. No. 0111-01; left) and Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01; right) followed by Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-BIOT (SB Cat. No. 6440-08, Streptavidin-CY3 (SB Cat. No. 7100-12), and DAPI.
  • ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-BIOT (SB Cat. No. 6440-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit IgG-UNLB isotype control (SB Cat. No. 0111-01; left) and Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01; right) followed by Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-BIOT (SB Cat. No. 6440-08, Streptavidin-CY3 (SB Cat. No. 7100-12), and DAPI.
 

ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-BIOT (SB Cat. No. 6440-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
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References
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