Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

Hamster Anti-Mouse CD3ε-UNLB (145-2C11)

Cat. No.:
1530-01
Purified Anti-Mouse CD3 epsilon antibody for use in flow cytometry, immunohistochemistry, western blot, immunoprecipitation, blocking, depletion, CMCD, and activation assays.
$135.00
Size Price (USD) Quantity
0.5 mg $135.00
More Information
Clone 145-2C11
Isotype Hamster (Armenian) IgG1
Isotype Control Hamster IgG-UNLB
Specificity Mouse CD3ε
Alternative Names CD3 epsilon
Description CD3ε, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The 145-2C11 monoclonal antibody recognizes an epitope on the 25 kDa ε chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble 145-2C11 can activate primed and naïve T cell in vitro. 145-2C11 can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL’s. Immobilized 145-2C11 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death.
Immunogen H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Flow Cytometry – Quality tested 1,10-12
Immunohistochemistry – Reported in literature 9
Immunoprecipitation – Reported in literature 1,8
Western Blot – Reported in literature 8
Blocking – Reported in literature 2,3
Depletion – Reported in literature 6
Activation – Reported in literature 1,4,7
Complement Mediated Cell Depletion – Reported in literature 5

RRID Number AB_2794798
Gene ID 12501 (Mouse)
Gene ID Symbol Cd3e (Mouse)
Gene ID Aliases AI504783; CD3; CD3epsilon; T3e
UniProt ID P22646 (Mouse
UniProt Name CD3E_MOUSE (Mouse)

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
or call 800.722.2255
  1. 1. Leo O, Foo M, Sachs DH, Samelson LE, Bluestone JA. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc Natl Acad Sci USA. 1987;84:1374-78. (Immunogen, IP, Activ, FC)
  2. 2. Bai Y, Liu J, Wang Y, Honig S, Qin L, Boros P, et al. L-selectin-dependent lymphoid occupancy is required to induce alloantigen-specific tolerance. J Immunol. 2002;168:1579-89. (Block)
  3. 3. Vossen AC, Tibbe GJ, Kroos MJ, van de Winkel JG, Benner R, Savelkoul HF. Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects. Eur J Immunol. 1995;25:1492-6. (Block)
  4. 4. Bergese S, Pelletier R, Vallera D, Widmer M, Orosz C. Regulation of endothelial VCAM-1 expression in murine cardiac grafts. Roles for TNF and IL4. Am J Pathol. 1995;146:989-98. (Activ)
  5. 5. Wagner DH, Hagman J, Linsley PS, Hodsdon W, Freed JH, Newell MK. Rescue of thymocytes from glucocorticoid-induced cell death mediated by CD28/CTLA-4 costimulatory interactions with B7-1/B7-2. J Exp Med. 1996;184:1631-38. (CMDM)
  6. 6. Han WR, Murray-Segal LJ, Gershenzon A, Zhang JG, Hodder AN, Pietersz GA, et al. Idarubicin-145-2C11-F(ab')2 promotes peripheral tolerance and reduces chronic vascular disease in mouse cardiac allografts. Transpl Immunol. 1999;7:207-13. (Depletion)
  7. 7. Silverio JC, Pereira IR, Cipitelli M, Vinagre NF, Rodrigues MM, Gazzinelli RT, et al. CD8+ T-cells expressing interferon gamma or perforin play antagonistic roles in heart injury in experimental Trypanosoma cruzi-elicited cardiomyopathy. PLoS Pathog. 2012;8(4):e1002645. (Activ)
  8. 8. Molnár E, Swamy M, Holzer M, Beck-Garcia K, Worch R, Thiele C, et al. Cholesterol and sphingomyelin drive ligand-independent T-cell antigen receptor nanoclustering. J Biol Chem. 2012;287:42664-74. (IP, WB)
  9. 9. Al-Falahi Y, Sand KL, Knudsen E, Damaj BB, Rolin J, Maghazachi AA. Splenic natural killer cell activity in two models of experimental neurodegenerative diseases. J Cell Mol Med. 2009;13:2693-703. (IHC)
  10. 10. Conrad ML, Davis WC, Koop BF. TCR and CD3 antibody cross-reactivity in 44 species. Cytometry. 2007;71A:925-33. (FC)
  11. 11. Bauler LD, Duckett CS, O'Riordan MX. XIAP regulates cytosol-specific innate immunity to Listeria infection. PLoS Pathog. 2008;4(8):e1000142. (FC)
  12. 12. Grodeland G, Mjaaland S, Roux KH, Fredriksen AB, Bogen B. DNA vaccine that targets hemagglutinin to MHC class II molecules rapidly induces antibody-mediated protection against influenza. J Immunol. 2013;191:3221-31. (FC, Grizzly Bear Reactivity)
See All References