ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA and human, bovine, and horse serum. Immunoglobulins and sera were detected with serially diluted Goat Anti-Mouse IgG2a, Human/Bovine/Horse SP ads-UNLB (SB Cat. No. 1083-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).