Rat Anti-Mouse CD49e-FITC
Cat. No.
1695-02
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Size
0.5 mg
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Price (USD)
$285.00
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Clone |
5H10.27
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Isotype |
Rat (Lewis) IgG2aκ
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Isotype Control |
Rat IgG2a-FITC
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Immunogen |
C57BL/6 x A/J F1 mouse mast cell line MC/9
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Specificity |
Mouse CD49e
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Alternalte Name(s) |
Integrin α5, VLA-5α
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Description |
The monoclonal antibody 5H10.27 reacts with the α5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines. Soluble 5H10.27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10.27 has also been demonstrated to costimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody.
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Format/Conjugate |
FITC (Fluorescein)
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Buffer Formulation |
Phosphate buffered saline containing 0.1% sodium azide
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Concentration |
0.5 mg/mL
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Volume |
1.0 mL
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Storage & Handling |
2-8°C; Avoid exposure to light
Please refer to product specific SDS
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Applications |
Applications for relevant formats of this clone include - Flow Cytometry – Quality tested 1,2 Immunohistochemistry-Frozen Sections – Reported in literature 3 Blocking – Reported in literature 1,2 Costimulation – Reported in literature 2 Adhesion – Reported in literature 1,2
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Recommended Dilutions |
Please refer to product specific Technical Bulletin
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RRID |
AB_2795144
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Gene ID |
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Gene Symbol |
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Gene Aliases |
Fnra; VLA5; Cd49e
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Uniprot ID |
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Uniprot Name |
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-
C57BL/6 mouse bone marrow cells were stained with Rat Anti-Mouse CD49e-AF647 (SB Cat. No. 1695-31).
C57BL/6 mouse bone marrow cells were stained with Rat Anti-Mouse CD49e-AF647 (SB Cat. No. 1695-31).
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References
1. | Kinashi T, Springer TA. Adhesion molecules in hematopoietic cells. Blood Cells. 1994;20:25-44. (Immunogen, FC, Block, Adhesion) | 2. | Rich S, Van Nood N, Lee H. Role of α5β1 integrin in TGF-β1-costimulated CD8+ T cell growth and apoptosis. J Immunol. 1996;157:2916-23. (Costim, Block, Adhesion, FC) | 3. | Schmieder AH, Caruthers SD, Zhang H, Williams TA, Robertson JD, Wickline SA, et al. Three-dimensional MR mapping of angiogenesis with α5β1(αVβ3)-targeted theranostic nanoparticles in the MDA-MB-435 xenograft mouse model. FASEB J. 2008;22:4179-89. (IHC-FS) |
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