Rat Anti-Mouse CD86-BIOT

Cat. No.
1915-08  
Size
0.5 mg  
Price (USD)
$222.00 
Clone 2D10
Isotype Rat (Lewis) IgG2bκ
Isotype Control Rat IgG2b-BIOT
Immunogen Mouse B cell lymphoma cell line 5C2
Specificity Mouse CD86
Alternalte Name(s) B7-2, B70, Ly-58
Description CD86 (B7-2) is an 80 kDa type I integral membrane protein and a member of the immunoglobulin superfamily of cell surface receptors. Along with CD80 (B7-1), CD86 participates in T cell costimulation via interaction with CD28 and CD152 (CTLA-4). It is expressed at very low density in normal tissues but is rapidly upregulated on stimulated B cells, monocytes, and dendritic cells. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,2,4,6,7
Immunohistochemistry-Frozen Sections – Reported in literature 4
Immunoprecipitation – Reported in literature 1
Blocking – Reported in literature 1-3,5
Separation – Reported in literature 2
Complement Mediated Cell Depletion – Reported in literature 2
ELISA – Reported in literature 3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795516
Gene ID
12524 (Mouse)
Gene Symbol
Cd86 (Mouse)
Gene Aliases B7; B7-2; B7.2; B70; CLS1; Cd28l2; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2
Uniprot ID
P42082 (Mouse)
Uniprot Name
  • DBA/2 mouse mastocytoma cell line P815 and mB7.2-Ig transfected P815 cells were stained with Rat Anti-Mouse CD86-FITC (SB Cat. No. 1915-02).

DBA/2 mouse mastocytoma cell line P815 and mB7.2-Ig transfected P815 cells were stained with Rat Anti-Mouse CD86-FITC (SB Cat. No. 1915-02).
 
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References
1. Chen C, Faherty DA, Gault A, Connaughton SE, Powers GD, Godfrey DI, et al. Monoclonal antibody 2D10 recognizes a novel T cell costimulatory molecule on activated murine B lymphocytes. J Immunol. 1994;152:2105-14. (Immunogen, FC, IP, Block)
2. Roth R, Nakamura T, Mamula MJ. B7 costimulation and autoantigen specificity enable B cells to activate autoreactive T cells. J Immunol. 1996;157:2924-31. (FC, Sep, CMDC, Block)
3. Bagenstose LM, Class R, Salgame P, Monestier M. B7-1 and B7-2 co-stimulatory molecules are required for mercury-induced autoimmunity. Clin Exp Immunol. 2002;127:12-19. (Block, ELISA)
4. Issazadeh S, Navikas V, Schaub M, Sayegh M, Khoury S. Kinetics of expression of costimulatory molecules and their ligands in murine relapsing experimental autoimmune encephalomyelitis in vivo. J Immunol. 1998;161:1104-12. (FC, IHC-FS)
5. Shanafelt M, Kang I, Barthold SW, Bockenstedt LK. Modulation of murine Lyme borreliosis by interruption of the B7/CD28 T-cell costimulatory pathway. Infect Immun. 1998;66:266-71. (Block)
6. Grodeland G, Mjaaland S, Roux KH, Fredriksen AB, Bogen B. DNA vaccine that targets hemagglutinin to MHC class II molecules rapidly induces antibody-mediated protection against influenza. J Immunol. 2013;191:3221-31. (FC)
7. Matsuo H, Yoshimoto N, Iijima M, Niimi T, Jung J, Jeong S, et al. Engineered hepatitis B virus surface antigen L protein particles for in vivo active targeting of splenic dendritic cells. Int J Nanomedicine. 2012;7:3341-50. (FC)