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  • Mouse Anti-Human IgM-UNLB (SA-DA4-R - Recombinant)

Mouse Anti-Human IgM-UNLB (SA-DA4-R - Recombinant)

Cat. No.:
29020-01
Purified Anti-Human IgM recombinant antibody for use in ELISA and flow cytometry assays.
$80.00
Size Price (USD) Quantity
0.25 mg $80.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone SA-DA4-R
(Genetically derived from DNA sequences of a parental hybridoma clone; for details on the parental clone, see Cat. No. 9020)
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-UNLB (15H6)
Specificity Human IgM
Immunogen Human IgM myeloma protein
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.5 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested>
Flow Cytometry – Quality tested

Gene ID 3507 (Human)
Gene ID Symbol IGHM (Human)
Gene ID Aliases AGM1; MU; VH
UniProt ID P01871 (Human)
UniProt Name IGHM_HUMAN (Human)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. Frozen newborn mouse cartilage section was stained with Mouse IgG<sub>1</sub>-UNLB isotype control (SB Cat. No. 0102-01; left) and Mouse Anti-Type II Collagen-UNLB (right) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-FITC (SB Cat. No. 1070-02) and DAPI.
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  2. ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgM-UNLB (SB Cat. No. 9022-01) followed by Goat Anti-Mouse IgG<sub>3</sub>, Human ads-HRP (SB Cat. No. 1100-05).
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  3. ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgM-UNLB (SB Cat. No. 9020-01) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-HRP (SB Cat. No. 1070-05).
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  4. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human ads-AF647 (SB Cat. No. 1070-31).
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  5. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  6. Human peripheral blood lymphocytes were stained with Goat Anti-Human IgM-BIOT (SB Cat. 2020-01) and Mouse Anti-Human CD19-PE (SB Cat. No. 9340-09) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-FITC (SB Cat. No. 6300-02).
    Goat Anti-Human IgM-UNLB
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  7. ELISA plate was coated with serially diluted Human IgM Lambda-UNLB (SB Cat. No. 0158L-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  8. Ramos cell lysates treated with HM71224 and stimulated with Goat F(ab')<sub>2</sub> Anti-Human IgM-UNLB (SB Cat. No. 2022-01) were resolved by electrophoresis, transferred to PVDF membrane, and probed with anti-phospho-PLCγ2Y1217 followed by an HRP conjugated secondary antibody and chemiluminescent detection.<br/>Image from Kim Y, Park KT, Jang SY, Lee KH, Byun J, Suh KH, et al. HM71224, a selective Bruton's tyrosine kinase inhibitor, attenuates the development of murine lupus. Arthritis Res Ther. 2017;19:211. Figure 1(a)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  9. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Rat Anti-Mouse IgG<sub>1</sub>-AF488 (SB Cat. No. 1144-30).
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  10. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Mouse IgG<sub>1</sub>, Human ads-AF555 (SB Cat. No. 1072-32).
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  11. ELISA plate was coated with purified human IgM, IgG, and IgA and mouse, bovine, and horse serum. Immunoglobulins and sera were detected with serially diluted Goat Anti-Human IgM, Mouse/Bovine/Horse SP ads-UNLB (SB Cat. No. 2023-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  12. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human/Bovine/Horse SP ads-HRP (SB Cat. No. 1073-05).
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